Journal: World Journal of Microbiology & Biotechnology

Article Title: Genomic and functional insights into the thermophilic strain Geobacillus sp. Geo 8.1: a source of thermostable xylanase for sustainable bioprocesses

doi: 10.1007/s11274-026-04840-1

Figure Lengend Snippet: The Circular Genome View Geobacillus strain Geo 8.1. The tracks on the viewer are displayed as concentric rings, from outermost to innermost: Position, Contigs/Chromosomes, CDS-forward, CDS-reverse, Non-CDS Features, GC Content, and GC Skew

Article Snippet: Geobacillus sp. Geo 8.1 , Geobacillus stearothermophilus ATCC 12,980 , 58,5 , [54,8–62,0] , 38,8 , [36,3–41,3] , 54,1 , [51,0–57,2] , 0,58.

Techniques:

Journal: World Journal of Microbiology & Biotechnology

Article Title: Genomic and functional insights into the thermophilic strain Geobacillus sp. Geo 8.1: a source of thermostable xylanase for sustainable bioprocesses

doi: 10.1007/s11274-026-04840-1

Figure Lengend Snippet: Phylogenetic tree inferred with FastME 2.1.6.1 (Lefort et al. ) using GBDP distances calculated from whole-genome comparisons. Branch lengths are scaled according to the GBDP distance formula d5, and the scale bar indicates substitutions per site. Numbers above branches represent GBDP pseudo-bootstrap support values > 60% from 100 replications, with an average branch support of 58.0%. The tree was rooted at the midpoint (Farris ). The coloured annotations on the right summarize genome-based metadata, including species and subspecies clusters defined by TYGS (dDDH thresholds of 70% and 79%, respectively), genomic G + C content (%), GBDP delta statistics, genome size (bp), predicted protein count, and SSU rRNA length (bp). The “User strain?” column marks the strain analysed in this study ( Geobacillus sp. Geo 8.1), whereas the “Type species?” column indicates the type strains included in the analysis. For quantitative colour-coded annotations, darker shades indicate higher values

Article Snippet: Geobacillus sp. Geo 8.1 , Geobacillus stearothermophilus ATCC 12,980 , 58,5 , [54,8–62,0] , 38,8 , [36,3–41,3] , 54,1 , [51,0–57,2] , 0,58.

Techniques:

Journal: World Journal of Microbiology & Biotechnology

Article Title: Genomic and functional insights into the thermophilic strain Geobacillus sp. Geo 8.1: a source of thermostable xylanase for sustainable bioprocesses

doi: 10.1007/s11274-026-04840-1

Figure Lengend Snippet: Thin-layer chromatographic (TLC) analysis of hydrolytic products obtained from crude enzyme extracts of Geo 8.1 incubated with birchwood xylan at 65 °C. Lane 1: reaction products after 20 min; lane 2: reaction products after 24 h; lanes 3–8: xylose, xylobiose, xylotriose, xylotetraose, xylopentaose, and xylohexose standards, respectively. The results show the progressive conversion of xylan-derived oligosaccharides to xylose, indicating the presence of both endo-xylanase and β-xylosidase activities

Article Snippet: Geobacillus sp. Geo 8.1 , Geobacillus stearothermophilus ATCC 12,980 , 58,5 , [54,8–62,0] , 38,8 , [36,3–41,3] , 54,1 , [51,0–57,2] , 0,58.

Techniques: Incubation, Derivative Assay

Journal: World Journal of Microbiology & Biotechnology

Article Title: Genomic and functional insights into the thermophilic strain Geobacillus sp. Geo 8.1: a source of thermostable xylanase for sustainable bioprocesses

doi: 10.1007/s11274-026-04840-1

Figure Lengend Snippet: Proposed xylan degradation and D-xylose catabolic pathway in Geo 8.1. Enzymes and their corresponding EC numbers are indicated along the pathway, and Geo 8.1 locus tags are provided in parentheses. The scheme illustrates the enzymatic steps from extracellular xylan hydrolysis to intracellular D-xylose catabolism via the pentose phosphate pathway and glycolysis

Article Snippet: Geobacillus sp. Geo 8.1 , Geobacillus stearothermophilus ATCC 12,980 , 58,5 , [54,8–62,0] , 38,8 , [36,3–41,3] , 54,1 , [51,0–57,2] , 0,58.

Techniques:

Journal: Acta biomaterialia

Article Title: Sex- and region-specific differences in microstructural remodeling and passive biomechanics of the aorta correlate with aneurysm propensity in a mouse model of severe Marfan syndrome

doi: 10.1016/j.actbio.2025.05.056

Figure Lengend Snippet: Representative en face multiphoton images of ASC for WT and MU, male and female mice. These images illustrate the qualitative changes in collagen fiber (red), elastic fiber (green), and cell nuclei (blue) organization in the medial and adventitial layers for each group. The multiphoton images for DSC, SAB and IAB are presented in – . Scale bars = 50 μm.

Article Snippet: Z-stacks of ASC, DSC, SAB and IAB were collected using a sp-8 DIVE multiphoton microscope (Leica) with a 60× objective and 2 μm step sizes.

Techniques: